Cell Death
Cell ablation, apoptosis and cell lysis can all be called cell death.
Apoptosis is a highly regulated independent response to environmental challenges that cause stress. Photogenetic analogues of apoptotic protein participants may be used to externally regulate life / death decisions with fine biochemical, spatial and temporal accuracy.
Cell ablation is a powerful method to clarify the contribution of a single cell population to embryonic development and tissue regeneration.
Optogenetic technology has the potential to achieve targeted cell death in a more accurate way.
As an expert in biotechnology, Creative BioMart insists on developing high-quality one-stop services in the field of optogenetics.
Based on our advanced molecular biology and photogenetics technology platform, we are committed to providing customers with customized photoinduced cell ablation, apoptosis and lysis solutions.
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Light induced apoptosis
Cysteine aspartate protein has been proved to be the executioner protein of apoptosis. The continuous activation of caspase plays a central role in the executive stage of apoptosis. Therefore, the last checkpoint in the caspase cascade is an ideal place to develop cell death control mechanisms.
We can regulate cell apoptosis by fusing the photosensitive LOV2 domain and the apoptosis executive domain in series. This technique can be used to study biological interactions and effects with high temporal and spatial accuracy.
Zebrafish cell target ablation
Cell ablation is a powerful method to clarify the contribution of a single cell population to embryonic development and tissue regeneration. Optogenetic technology has the potential to achieve targeted cell ablation in a more accurate way. We express inducible phototoxic proteins in specific cells of interest through transgenic technology, so that local application of low-power light with the correct wavelength can induce apoptosis.
For example, KillerRed protein is such a gene encoded photosensitizer. When irradiated with 520-590 nm light in the presence of oxygen, excess reactive oxygen species (ROS) will be produced in membrane bound killerred expressing cells, resulting in apoptosis within one hour
Cyanobacteria cell lysis
CcaS is a sensor histidine kinase, which can activate its homologous response regulator CcaR under green light to induce the gene expression. Under red light, CcaS is in the "off state" and the expression of target genes is not activated. However, under the green light, CcaS is in the "on state", resulting in the activation of CcaR by phosphorylation and the simultaneous induction of target gene expression. Through the above mechanism, the regulation of cell lysis by green light can be realized.
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