CRY2 is a FAD containing photoreceptor from Arabidopsis. Arabidopsis cryptochrome 2 (CRY2) can simultaneously carry out light dependent CRY2-CRY2 homooligomerization and CRY2-CIB1 heterodimerization, both of which have been widely used to optically control intracellular processes. Both configurations can be activated by blue light. Solutions and services based on both switch systems are now available at Creative BioMart.

Basic Principle of CRY2 Activated Mammalian System

In Arabidopsis, CIB1 (basic helix loop helix of cryptochrome interaction) was identified to interact with CRY2 in a blue light specific manner to promote flower initiation. This light induced heterodimerization of CRY2-CIB1 is mapped to the PHR domain of CRY2 and then used for the optical control of protein-protein interaction.CRY2-CIB1 system has been successfully used for intracellular signal light control, lipid metabolism, gene expression and organelle transport, and has been found to function robustly in a variety of organisms without the need for exogenous cofactors.

Available Services at Creative BioMart

CRY2-Tet Activated Mammalian System

A Light & Tet Operon Dually Controlled System (Light/Tet System for short) construction service is now available. The system integrates the advantages of light and chemical induction, and can accurately regulate the expression of target genes at single-cell resolution.

CRY2 Activated CHO Cell Line Construction Services

CRISPR-dCas9 system controlled by CRY2/CIB1 optogenetic switch can be used to regulate transient gene expression. This CHO expression system construction service based on optogenetic switch is now accessible

Arabidopsis cryptochrome Expression Services

The lack of a suitable protein expression system to obtain the whole protein of cryptochrome with photochemical activity is a technical obstacle in the research of plant cryptochrome. Creative BioMart provides a one-stop service to express Arabidopsis Cryptochrome in HEK293T cells for follow-up research by scientific research institutions.

CRY1 and CRY2 Knockout Cell Line Construction Services

The duplex CRISPR/Cas9 gene editing strategy aims to delete the entire exon by introducing double strand breaks simultaneously in the two intron regions flanking the exon intron connection. We can construct CRY1 knockout cells, CRY2 knockout cells and CRY1/CRY2 double knockout cells using CRISPR/Cas9 technology.

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

Reference

1. Liu, H., Liu, B., Zhao, C., Pepper, M., & Lin, C. (2011). The action mechanisms of plant cryptochromes. Trends in plant science, 16 12, 684-91 .

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