Photogenetics is a new field with rapid development. With the in-depth study of photoreceptor proteins in the field of botany, photogenetics technology has begun to use the optical switching activity of plant photoreceptors to control intracellular signal pathways and applied in the fields of synthetic biology.

UV-B resistance 8 (UVR8) is an UV-B sensing protein found in Arabidopsis thaliana. UV8 is responsible for sensing ultraviolet rays in the range of 280-315 nm and initiating plant stress response. It is most sensitive at 285nm, close to the lower limit of UVB.

UV receptors is one of the optogenetics switches available at Creative BioMart. We provide plenty of services and related solutions based on the UVR8/UVR8 and UVR8/COP1 switch system.

UVR8/UVR8 and UVR8/COP1 Switch System

UVR8/UVR8

In the absence of UV-B light, UVR8 is inactive as a homodimer. Under UV-B light irradiation, the internal tryptophan residues in UVR8 will sense the light signal, and the protein will be activated by light through dimer to monomer conversion within a few seconds.

UVR8/COP1

Constitutive light signal regulator 1 (COP1) is a central light signal regulator that can interact with monomeric UVR8 in a few minutes to initiate UV-B light signal transduction in plant cells. The physical interaction between UVR8 and COP1 mainly depends on the C-terminal VP motif of UVR8 and the C-terminal WD40 domain of COP1.

Why Select UV-B Switch System?

• UVR8 photogenetics tools can be applied to non-plant systems without synthetic chromophores.
• The specific wavelength absorbed by UVR8 can perform polychromatic imaging of fluorescent labeled proteins that have been widely used without interfering with UVR8 photoactivation.
• High sensitivity, allowing photogenetic stimulation at light levels well below the safe threshold for plant, yeast and mammalian cell proliferation.
• UV-B is a fast and powerful switch for photogenetic regulation.
• After removing UV-B light, monomer UVR8 is reversed to homodimer state in vivo to realize light switchable control.
• COP1 responds to UV-B light and promotes the nuclear accumulation of UVR8. It provides potential for strategies involving the control of protein nuclear localization by UV-B light.

What We Can Do For You?

Our services and solutions based on the UVR8/UVR8 and UVR8/COP1 switch system include but not limited to

• Services

UVR8/UVR8 Controlled Mammalian Secretion System Construction Service

UVR8/COP1 Controlled Mammalian Cell Line Construction Service

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

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