Acute kidney injury (AKI) is associated with high mortality and incidence rate. AKI attacks can lead to chronic kidney disease and end-stage renal disease. Despite therapeutic interventions, there are no approved drugs for the treatment and prevention of AKI. The unsuccessful development of AKI treatment strategies can be attributed to the insufficient understanding of the pathophysiology and adverse drug reactions of AKI. Therefore, there is a clear need for sustained efforts to master the complex pathophysiology and innovative therapies of AKI.
Recently, it has been reported that the nervous system plays an important role in the pathophysiology of various kidney diseases, including AKI, directly or through the immune system. Photogenetics is a pioneering technology in the field of neuroscience. It helps to stimulate or inhibit specific neurons by light.
Creative BioMart provides various solutions for clients who desire to do acute kidney injury therapeutic research. We have the ability to provide the construction services of cell lines, expression systems and model organisms.
Viral vector design and delivery
For in vivo experiments, viral vectors or transgenic mice are often used to introduce ChR2 into specific cell types. The specific expression of ChR2 is achieved by injecting the viral vector into the target region and/or using the Cre/lox p system.
Construction of the ChR2 Switch System For Therapeutic Research
A new technology called optogenetics can optically control cells in living tissue, usually neurons, which are genetically modified to express photopsin. Channelrhodopsin-2 (ChR2) is a opsin, a non selective cation channel residing in the cell membrane. After exposure to monochromatic light of blue wavelength, it will quickly open its door. Unlike electrodes, blue light can selectively depolarize neurons expressing ChR2, mainly through Na+ entry to induce action potentials. The use of ChR2 and several variants to regulate kinetic properties and photogenetics of inhibitory opsin helps to understand the role of the nervous system in AKI, leading to the clinical application of neuromodulation in AKI treatment.
Construction of kidney injury mice model for therapeutic strategy development
Photogenetics may be a useful tool to study the mechanism of vagus nerve stimulation (VNS) protecting kidney. Vagal afferent neurons were found to be divided into several subgroups according to their specific markers. The optogenetic stimulation of each subgroup affects the functions of gastrointestinal tract, lung and heart in different ways. These studies suggest that some subgroups of vagal afferent and possible efferent neurons may play an important role in the renal protection of VNS. In addition, photogenetic stimulation of specific neurons in the brain can improve ischemia-reperfusion injury in mice
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