The production of recombinant protein is highly technical and requires strong professional knowledge, because one production scheme can not be suitable for all product types. With years of experience and the expression of 1000 + proteins, Creative BioMart has developed an internal scheme to include up to 32 expression condition tests to meet your best requirements. The technical possibilities are almost unlimited, and such a lasting experience is decisive.
We have mature protein expression system and advanced protein expression technology. If necessary, we recommend that you choose this service as an ancillary service of the optogenetic expression system.
We are devoting ourselves to improve your opportunities to effectively produce recombinant protein. Our protein production service in E.coli includes up to 32 standard protein expression tests to ensure that even the most difficult to express proteins have high production potential.
| Our test rang include | Details |
| E. coli strains | Customer can select up to 4 different strains for expression test, and we will score the expression level of each strain |
| Temperature | Typically, we will conduct test the expression at 30 ℃ and 37 ℃. If you have other temperature requirements, please inform us at the beginning of the project. |
| Induction time | Generally, we conduct induction test for 1 h and 4 h. The induction time may varies slightly according to the strain. If you have other requirements, please inform us at the beginning of the project. |
| Protein extraction method | If required, we will choose several protein extraction methods according to the properties of the protein. |
| Purification method | We will choose two purification methods according to the properties of the protein. |
All conditions are varied from codon optimizations, vectors with different promoters, protein sequences, purification tags down to the purification method itself, tag clivage conditions, refolding method and formulation. For difficult to express proteins we also have a dedicated high throughput screening service that allow to increase the probability to find the most optimal conditions. The more conditions are tested, the higher the probability to ensure optimal solubility, functionality, productivity.
You need to produce a protein in E. coli or want more information about our capacities of protein expression in E. coli? Our account manager is at your disposal to answer to your questions. Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
