The intracellular transport and distribution of organelles play an important role in a variety of cell functions, including cell polarization, intracellular signal transduction, cell survival and apoptosis. Through the dimerization of CRY2-CIB1, a common optical switch, the precise spatio-temporal control of light on the transportation and distribution of organelles can be realized. Creative BioMart provides customers with customized cell line construction services. At present, it can realize the photoinduced driving and aggregation behavior of mitochondria, peroxisomes and lysosomes.

Figure 1: Optogenetic control of molecular motors and organelle distributions in cells (Duan L, 2015)

Technical Principle

CRY2 sequences were anchored to organelles through specific organelle targeting transmembrane domains by editing techniques.

Scheme 1: CIB1 is associated with truncated kinesin. Under blue light irradiation, cib1-cry2 binds to recruit the kinesin motor to the organelle, which drives the organelle to move to the positive end of the microtubule.

Scheme 2: CIB1 domain is linked to dynein / dynein adaptor protein. When irradiated with blue light, cib1 binds to CRY2 to recruit dynein into organelles. Therefore, organelles are pushed to the negative end of microtubules.

Figure 2: Schematic of light-controlled motor recruitment and organelle transport in cells (Duan L, 2015)

Applications

• Mitochondrial redistribution induced by light flux
• Photoinduced relocation of peroxisome
• Molecular motors recruited by light move along microtubules
• Photogenetic control of lysosomal cargo distribution

Why Choose Creative BioMart

• CRY2 and CIB1 dimerize in sub seconds after exposure to blue light, which does not require exogenous ligands and low-intensity light.
• Light induced motor recruitment and organelle movement are repeatable and reversible, and can be realized in subcellular regions.
• This cell line is expected to provide a new strategy for studying the causal role of organelle transport and distribution in the function of living cells.
• Creative BioMart has been upgrading and innovating existing technologies to ensure satisfactory delivery time and results.
• We provide cost-effective one-stop service, and the whole experimental process is transparent and verifiable.
• We provide customized services for each customer and formulate experimental schemes according to customer requirements.
• We have an advanced technical team and experimental platform, and all technicians have been strictly trained and screened to ensure the most accurate experimental results.
• We strictly keep business secrets and never disclose customer information.

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

Reference

1. Duan L, Che D, Zhang K, Ong Q, Guo S, Cui B. Optogenetic control of molecular motors and organelle distributions in cells. Chem Biol. 2015 May 21;22(5):671-82.