The optical control of protein-protein interaction has been proved to be a valuable tool in many fields of neuroscience, cell and developmental biology. The wide adoption of optical control systems stems from the excellent spatio-temporal control level provided by these systems. At present, the development of optical switching tools mainly depends on small-scale library screening to optimize the coupling between optical switching proteins and their fusion partners.
At present, the sequence space of each pair of photoswitchable protein partner has not been explored to a great extent. Changes in the surface of photoswitchable protein partner may produce engineering pairs with improved properties (such as higher affinity or selectivity), and may make the development of orthogonal interaction pairs possible.
Creative BioMart characterized the photoswitchable protein-protein interaction library by yeast two hybrid (Y2H) method. Y2H results reflect the affinity, expression, optical switching and chromophore uptake of protein-protein interactions, and are well correlated with the results obtained in vitro and mammalian cells.
The purpose of the tool is to evaluate the photoswitchable protein-protein interaction library using Y2H selection and screening.
After the protein (bait) you are interested in physically binds to the protein fragment (prey) in the library, the DNA binding domain (DBD) of Gal4 will be close to its activation domain (AD). Y2H results will reflect the affinity, expression, optical switching and chromophore uptake of protein-protein interactions.Therefore, by selecting systematic changes in parameters, Y2H screening can be effectively used to generate new photogenetic tools for controlling protein-protein interactions in different environments.
We have extensive experience in handling dual hybrid systems. At the same time, there are a large number of prefabricated cDNA libraries for you to choose from.
We optimize the classic dual hybrid protocol and provide customers with specific customized solutions according to their different purposes.
We have more than 10 years of working experience in two hybrid research and experimental operation skills.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
