Current diabetes treatments include the use of drugs that increase insulin production or direct injection of insulin to manually regulate blood glucose levels.  Both of these conditions can lead to poor blood glucose regulation, and blood glucose levels often peak and decline. The new method of photogenetics can realize the identification of intact islets β Cell specific studies. Establish a process in which proteins change their activity in response to light to develop a new method to increase insulin production while maintaining a real-time link between insulin release and blood glucose concentration.

Creative BioMart provides various solutions for clients who desire to do diabetes and insulin research. We have the ability to provide the construction services of cell lines, expression systems and model organisms.

Optogenetic Tools for Diabetes Research

Tools that enables specific investigation of β cells in intact islets

Insulin from the pancreas β Cell release is triggered by glucose metabolism, resulting in membrane depolarization, opening of voltage dependent Ca²⁺ channels and further Ca²⁺ dependent exocytosis. The photosensitive cation channel channelrhodopsin-2 (ChR2) was expressed under the insulin promoter to establish transgenic mice. Stimulation of ChR2 with blue LED light triggered a rapid increase in intracellular Ca²⁺. 

Pancreas β Cells for photogenetics

The cell is loaded with a gene encoding photoactivated adenylate cyclase (PAC) enzyme. PAC produces cyclic adenosine monophosphate (cAMP) when exposed to blue light. When blood glucose concentration is high, it mOptogenetic Tools for Pain Researchakes β  Insulin production in the cells was tripled. Insulin production did not increase in the absence of glucose.

When exposed to blue light, these engineered pancreatic cells can be transplanted into the skin of diabetic mice, which can improve glucose tolerance, reduce hyperglycemia and raise plasma insulin level.

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

Reference

1. Kushibiki T, Okawa S, Hirasawa T, Ishihara M. Optogenetic control of insulin secretion by pancreatic β-cells in vitro and in vivo. Gene Ther. 2015 Jul;22(7):553-9.

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