Current diabetes treatments include the use of drugs that increase insulin production or direct injection of insulin to manually regulate blood glucose levels. Both of these conditions can lead to poor blood glucose regulation, and blood glucose levels often peak and decline. The new method of photogenetics can realize the identification of intact islets β Cell specific studies. Establish a process in which proteins change their activity in response to light to develop a new method to increase insulin production while maintaining a real-time link between insulin release and blood glucose concentration.
Creative BioMart provides various solutions for clients who desire to do diabetes and insulin research. We have the ability to provide the construction services of cell lines, expression systems and model organisms.
Tools that enables specific investigation of β cells in intact islets
Insulin from the pancreas β Cell release is triggered by glucose metabolism, resulting in membrane depolarization, opening of voltage dependent Ca2+ channels and further Ca2+ dependent exocytosis. The photosensitive cation channel channelrhodopsin-2 (ChR2) was expressed under the insulin promoter to establish transgenic mice. Stimulation of ChR2 with blue LED light triggered a rapid increase in intracellular Ca2+.
Pancreas β Cells for photogenetics
The cell is loaded with a gene encoding photoactivated adenylate cyclase (PAC) enzyme. PAC produces cyclic adenosine monophosphate (cAMP) when exposed to blue light. When blood glucose concentration is high, it mOptogenetic Tools for Pain Researchakes β Insulin production in the cells was tripled. Insulin production did not increase in the absence of glucose.
When exposed to blue light, these engineered pancreatic cells can be transplanted into the skin of diabetic mice, which can improve glucose tolerance, reduce hyperglycemia and raise plasma insulin level.
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