Pain is a widespread clinical problem. The conduction pathway of pain involves multiple levels in the periphery, spinal cord and spinal cord, which has the characteristics of complexity and diversity. To solve the problem of pain, we must first have a clear understanding of pain sensory pathway and neurobiological mechanism. Optogenetic is a technology that can selectively activate specific types of neurons in vivo. The development of this technology makes it possible to deeply analyze a variety of conduction pathways and regulatory mechanisms of the nervous system.
Creative BioMart provides various solutions for clients who desire to do pain research. We have the ability to provide the construction services of cell lines, expression systems and model organisms.
Optogenetic can selectively activate specific pain related neuronal subtypes in vivo, but does not provide strong basic research support for finding new targets in the process of pain information transmission and developing specific pain drugs. It is unknown that optogenetic technology has been applied to the study of pain conduction pathways at peripheral pain level, spinal cord pain level and above spinal cord level.
Tools for Nociceptors Research
Photogenetic stimulation of specific peripheral nociceptors is an important means to study different peripheral nociceptor subtypes and related spinal cord pathways. The channelrhodopsin-2 mouse model can be used to accurately stimulate specific nociceptor subsets in the experiment after gene site-specific knock-in, which is very helpful to understand the relationship between peripheral nociceptors and spinal cord neurons.
Tools for level regulation of spinal cord pain
Stereoscopic spinal cord slices can be used as experimental objects for research. For example, VGLUT3 CRE mice and cre dependent channelrhodopsin-2 mice were hybridized to specifically express channelrhodopsin-2 in VGLUT3 neurons, and then the postsynaptic effects induced by VGLUT3' nerve endings could be recorded in the dorsal horn of the spinal cord by preparing spinal cord slices preserving DRG and spinal nerve and stimulating DRG with laser.
New photogenetic tools
Traditional optogenetic tools need to rely on the expression of exogenous light sensitive molecules in the target system, which makes the application of this method complex and cumbersome in vivo. It is now possible to produce light sensitive properties for specific cell types through the application of compounds.
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