Reversible light base silencing is a key requirement to study the role of gene defined cell population, such as exploring the role of a single neuron in controlling network activity and behavior, or studying the effect of local heart block. K+ conductance is the basis of excitable cell resting state, so their activation can produce strong inhibition without significant changes in transmembrane ion gradient. Here, Creative BioMart provides a reversible silencing system based on potassium ion channels. This silencing service is generally applicable to cardiomyocytes and different types of neurons in mice and zebrafish.
PAC is a blue light activated cyclase, which is composed of blue light receptors using Flavin domain and coupled to cyclase domain through connector. They are identified in various species and provide a range of light sensitivity and enzyme kinetics.
The small cAMP gated K+ channel SthK from thermophilic Spirulina is well expressed in mammalian cells, and it carries a very large single channel current to ensure strong repolarization or hyperpolarization, making it the most promising candidate for silencing system.
We expressed SthK channel and small PAC in cell lines mediated by adenovirus to obtain strong photoactivated K+ current. A two-component optical silencing system composed of photoactivated adenylate cyclase and small cyclic nucleotide gated potassium channels. Activation of this silencing system by short low-intensity blue light pulses can lead to strong and reversible silencing of cardiomyocyte excitation and neuronal discharge.
The silencing service is generally applicable to cardiomyocytes and different types of neurons in mice and zebrafish.
Explore the role of a single neuron in controlling network activity and behavior.
To study the effect of local heart block area.
We have many years of experience in virus induced gene silencing services. We ensure that each specific experimental step is strictly controlled to ensure the high quality of virus induced gene silencing technology.
| Step | Description |
| Service consultation and discussion |
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| Vector construction |
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| Recombinant vector transformation |
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| Target cell infection | Cell cultivation and analysis |
| Result analysis | Silence efficiency analysis |
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