Light Controlled Mammalian System

Light Controlled Mammalian System

Chemically controlled molecular switches have played a central role in the emergence of synthetic biology and have become a conventional tool for mammalian cell biology research. However, the inherent disadvantages of molecules, such as difficulty in removing inducers and diffusion-based transport, hinder rapid reversibility and spatially resolved activation. On the other hand, these limitations have recently been addressed through the development of optogenetics, gene coding and light response tools, which can be controlled with unprecedented optical spatio-temporal accuracy.

The first breakthrough in mammalian optogenetics was the discovery of microbial opsin and its subsequent introduction into neuroscience. This hairstyle completely changed the study of neural networks. Later, beyond neuroscience, scientists have developed more optogenetic tools to control a variety of signal transduction processes in mammalian cells.

Creative BioMart has established a complete platform of optogenetic tools designed to control the life processes of different mammalian cells.

Overview of currently available optogenetic tools for mammalian systems

Light Photoreceptor Interaction partner Co-factor Mechanism Applications
UVB UVR8
UVR8
- - Homodimerization Protein secretion
COP1 - Heterodimerization Nuclear import Gene expression Chromatin targeting
Violet/ blue Dronpa - - Homodimerization Membrane recruitment/cell shape
Proteolytic cleavage
Blue LOV2 (from phototropin 1) - FMN Uncaging of C-terminal fusions Membrane recruitment/ Cell shape
Tunable protein dimerization
Nuclear import
Protein degradation
CRY2 CIB1 FAD Heterodimerization Membrane recruitment
Gene expression
Histone modification
Kinase activation
Phosphoinositide metabolism
Reversible protein inactivation
CRY2 - Oligomerization Signaling modulation
Kinase activation
EL222 - FMN Homodimerization Gene expression
FKF1 GI FMN Heterodimerization Membrane recruitment/cell shape
Gene expression
Melanopsin - 11-cisretinal Ca2+ influx Gene expression
PAC - FAD cAMP synthesis Signaling modulation
VVD - FMN Homodimerization Gene expression
Red/ far red PhyB PIF6 Phycocyanobilin Heterodimerization Membrane recruitment/cell shape
Signaling modulation
Gene expression

Why Select Optogenetic Tools for your Mammalian Cell Operation

Light Controlled Mammalian System

Timing

Photogenetics provides a high temporal resolution because receptors can be simply turned on and off according to the presence or absence of light of the correct wavelength. This response is immediate, in milliseconds, and can accurately associate neuronal activation with behavior.

Targeted manipulation

Photogenetic tools can target their expression to specific tissues, cell types and even subcellular regions of neurons.

Control stimulation

Some studies rely on providing reversible or different amounts of stimulation to receptors. Optogenetics can better control these factors because light sources can be easily and quickly manipulated or turned off.

Invasiveness 

Photogenetic tools need to be delivered to cells, usually through AAV viruses.

Related Services and Solutions

UVB Controlled Mammalian Expression System

UVB Triggered Protein Secretion System

CRY2 Activated Mammalian System

Red Light Activated Mammalian System

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

Reference

  1. Mansouri, M., Strittmatter, T., & Fussenegger, M. (2018). Light-Controlled Mammalian Cells and Their Therapeutic Applications in Synthetic Biology. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(1), 1800952.

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