Endotoxin Removal Service
Endotoxin is lipopolysaccharide (LPS) in the cell wall of Gram-negative bacteria. Endotoxin can cause strong immune response. We recommend that you add the endotoxin removal and detection service of Creative BioMart to your protein expression service package. We will provide effective endotoxin removal solutions, which can significantly reduce the endotoxin level in the production of recombinant protein, so as to meet the strict endotoxin control requirements of customers.
Work Flow of the Endotoxin Removal Service
Creative BioMart provides one-stop low endotoxin protein production solutions, including protein expression and purification and endotoxin removal services. The whole protein expression process was strictly controlled. All instruments and reagents used in the experiment will remove endotoxin to ensure low endotoxin level in the environment.
| Service step |
Description |
| Project evaluation and program building |
- Evaluation of target protein
- Evaluation of endotoxin removal efficiency and protein loss rate
- Formulate the scheme
|
| Endotoxin removal |
Endotoxin removal by customized process |
| Endotoxin quantitative test |
- Quantitative detection before and after endotoxin removal
- Tachypleus amebocyte lysate test
|
Endotoxin Removal Technology We Use
Creative BioMart has developed a series of methods to remove endotoxin. After the project evaluation, we will draft a customized endotoxin removal scheme for your target protein, using one method or a combination of methods to ensure the highest efficiency.
| Technology |
Endotoxin removal efficiency |
Protein recovery rate |
| Ion exchange chromatography |
99%. Low cost, high adsorption capacity. |
At least 80% |
| Affinity chromatography |
90%-98%.High efficiency and selectivity. |
40%-85% |
Different Levels of Endotoxin Removal Services
We provide different levels of endotoxin removal services to meet the needs of customers.
| Endotoxin removal level |
Detail |
| Low |
Endotoxin ≤1EU/μg |
| Middle |
Endotoxin ≤0.1EU/μg |
| High |
Endotoxin ≤0.01EU/μg |
If necessary, we can also use other endotoxin removal methods like liquid phase separation method, molecular sieve method, ultrafiltration method, adsorption method, distillation method, hydrophobic chromatography method.
Why Select Us?
- Satisfactory endotoxin removal effect. The endotoxin level of the product delivered can be as low as 10EU / mg
- Flexible endotoxin removal solutions.We provide different levels of endotoxin removal services to meet the needs of customers.
- High success rate.
- One-stop service from codon optimization to endotoxin removal.
- Predictable results. The technical team will estimate the protein loss rate at the beginning of the project to ensure that sufficient low endotoxin protein is provided.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
