Optogenetic-T7 E.coli system
The application of chemical inducers in space and time is limited. Spatiotemporal control has attracted more and more attention because biological systems are dynamically regulated and respond to intracellular stimuli and changes in internal state. Because of its precise temporal and spatial resolution, light has become the control input of biological systems.
Creative BioMart provides a light induced T7 RNA polymerase system to achieve precise regulation of transcription. Presently, we have not only established a complete R & D and design platform, but also established an experienced technical team.
Technology Principle
We photoinduced T7 RNA polymerase by dividing the polymerase into two fragments and fusing them into a light-activatable dimerization domain. After light induction, the two domains dimerize, so that the N-end of one domain is spatially close to the C-end of the other binding domain. This allows these domains to be fused to the C-terminal of the N-terminal fragment of the mitotic protein and the N-terminal of the C-terminal mitotic fragment, and the enzyme is spatially reconstructed by incorporating photogenetic regulation into the T7 RNA polymerase and assembled and dissociated by light stimulation.
Available Services at Creative BioMart
We provide construction services and protein expression services based on optogenetic-T7 E.coli system, including codon optimization, gene synthesis, protein expression and two-step purification. If you need other services, such as protein refolding, label removal and endotoxin removal services, you can also make additional choices. Through our proprietary codon optimization software and our experienced scientific team, we guarantee the delivery of high-quality final products.
| Service Name |
Service Description |
Deliverable |
| Preparation of the vector |
- Digestion with restriction enzyme, dephosphorylation.
- Gel purification
|
- Cloning vector
- QC data
- Final report
- Protein product( quantity as per customer requirements)
|
| Preparation of target DNA |
- Codon optimization
- Plasmid purification
- Gel purification
|
| Subclone construction |
- Subclone the target sequence to the pET vector
- Transformation of non-expression host bacteria
- Screening positive clones
- Clone sequencing to determine ORF
|
| Protein expression |
- Transformation of expression host E. coli strain that contains T7 RNA polymerase gene
- Plasmid stability test (optional)
- The cellular and subcellular components, expression time, temperature conditions, protein solubility and activity test
- Qualitative and quantitative analysis of protein products
|
| Scale up and protein purification |
- Amplification Culture
- Preparation of crude extract
- Affinity purification
- Cut off fusion tag and remove protease (if necessary)
|
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
