Saccharomyces cerevisiae, also known as budding yeast. It is generally spherical, oval and oval, and some are cylindrical and lemon shaped. Budding yeast Saccharomyces cerevisiae has become the primary eukaryotic model organism in the study of cell and molecular biology. Synthetic biology has adopted yeast as a biological platform for different purposes, including
As a microbial cell factory, yeast can be cultured in low-cost medium. It has the characteristics of fast growth, high protein yield, target protein secretion and post-translational modification.
In the past few years, light has become a widely used tool to interrogate and manipulate biological systems. Light has been proved to be an effective gene expression inducer, which can be quickly and easily applied or removed from the culture medium. In addition, it is cheap and highly adjustable, and can carry out fine temporal and spatial control of gene expression. The implementation of these switches overcomes the use of traditional chemical inducers, allows precise control of gene expression at a lower cost, leaves no chemical traces, and has a positive impact on the production of high-value metabolites and heterologous proteins.
Creative BioMart has been engaged in microbial gene editing for many years and has a perfect Saccharomyces cerevisiae gene editing technology platform. Our gene editing technology platform has experienced technicians, efficient experimental process and perfect experimental equipment. Our scientists can customize one-to-one solutions according to the specific needs of customers, and provide editing services for a variety of microorganisms to meet the needs of customers.
Based on PhyB/PIF3 interaction, the system can work as a change-over switch, and the on / off state is controlled by red / far red light. The system is based on the yeast two hybrid system as a fission transcription factor. We connected photoreceptors and their interacting proteins to the transcriptional activation elements of target genes through gene editing technology.
Similar to the red light optogenetic switch. Under light stimulation, the interaction between these proteins reconstructs a functional transcription factor that activates the expression of genes of interest (GOI).
Combined with optogenetic switch and crispr-cas9 technology. This configuration is designed to increase GOI expression.
Photoswitchable Protein Partner Libraries Screening Service
Photoswitchable Proteins Phage Display
Saccharomyces cerevisiae Gene Editing Service
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