Optogenetics is a technology of manipulating neural activities defined by genes with light. It has two main advantages: millisecond time accuracy and genetic spatial accuracy. Optogenetic technology has had a significant impact on neuroscience research by controlling specific types of cells in the intact brain. By implanting optogenetic elements (or using their own light sensing proteins) into the target site, the precise space-time control of the target can be realized in vivo and in vitro.
As a pioneer in the field of optogenetics, Creative BioMart is gradually improving our optogenetics equipment platform.
For optogenetic experiments of free behavior, it is necessary to transmit light to the brain as the animal moves. This is achieved by surgically implanting an optical cannula into a brain region that expresses an optogenetic probe. Select the appropriate length to reach the target brain region. After implantation, the light source will be connected through optical fiber cable, and the area under the sleeve will be illuminated.
Photogenetics can be carried out during microscopic experiments and synchronized with other microscopic methods. The device is suitable for the research of non free moving objects.
One strategy is to integrate optogenetics into a standard fluorescence microscope to detect the activity of nerve cells in target cells. For cell biology, optogenetics is integrated into their confocal imaging experiments to detect different genetic disturbances.
Under our setup. The collimating light source will be connected to the back falling fluorescence port to illuminate the sample. Depending on the light source, it will be connected to the rear port through direct coupling or to the falling fluorescence port through light guide / optical fiber. The light will propagate through the falling fluorescence port to the filter turntable, where it will be guided to your sample through the objective lens.
Our equipment has high-power LED light source, which is suitable for different optogenetic probes from ultraviolet to near infrared.
LED Light Source
LED is the first choice of photogenetics experiment, which can be used for (1) wide field photogenetics on microscope; (2) Experimental study on optogenetics of freely movable objects. First of all, the low cost, eye safety and long service life of LED make it an excellent choice for any laboratory that wants to carry out photogenetics. LED has a wide wavelength range and is suitable for different optogenetic probes from ultraviolet to near infrared. LEDs can be turned on and off at microsecond timing through pulse protocol. LEDs are easily triggered by TTL to synchronize pulses with external devices.
Laser Source
Lasers are commonly used in optogenetic experiments that require higher intensity and / or tighter focusing. Lasers provide very high intensity in a single spot, so they are usually used in laser scanning applications.
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