As a single-cell eukaryote, Saccharomyces cerevisiae not only has the most basic characteristics of all eukaryote life activities, but also has many advantages, such as clear background, rapid growth, convenient operation and so on. In fact, many regular understandings in modern genetics, cell biology and biochemistry are studied by yeast as experimental materials.
Creative BioMart has been engaged in microbial gene editing for many years and has a perfect Saccharomyces cerevisiae gene editing technology platform. Our gene editing technology platform has experienced technicians, efficient experimental process and perfect experimental equipment. Our scientists can customize one-to-one solutions according to the specific needs of customers, and provide editing services for a variety of microorganisms to meet the needs of customers.
The traditional method of gene knockout in Saccharomyces cerevisiae is to use the RecA and RecBCD proteins encoded by its own rec a homologous recombination system to mediate the homologous recombination of DNA. However, gene targeting based on this system has many disadvantages: firstly, because RecBCD has exonuclease activity, the linear targeted DNA will be degraded, The target gene must be integrated into the wearer for homologous recombination; Secondly, the target fragment needs a long homologous arm, often up to hundreds of bases, and the homologous recombination efficiency is low, so the required recombinants can not be obtained.
Crispr-cas9 gene editing technology is through artificially designed sgRNA (Guide RNA) to identify the target genome sequence, and guide cas9 protease to effectively cut DNA double strand to form double strand breaks. After damage, repair will cause gene knockout or knock in, and finally achieve the purpose of modifying genomic DNA. Compared with the traditional homologous recombination knockout gene method, crispr-cas9 gene editing technology is faster, traceless and more accurate Accurate, very convenient for you to carry out subsequent experimental research.
| Service | Description | Time |
| Gene knockout | Customer offers:
|
5-8 weeks |
| Gene knock in or point mutation | Customer offers:
|
5-8 weeks |
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
