Epigenetic States
Epigenetic refers to the changes of gene expression level based on non gene sequence changes, such as DNA methylation and chromatin conformation changes. The dynamic characteristics of gene expression make it possible for cell programming, homeostasis and environmental adaptation in life system. Analyzing the function of causal genes in the process of cells and organisms requires methods that can accurately regulate gene expression in space and time. At present, a variety of microbial and plant derived photosensitive proteins are designed as optogenetic drivers, which can control the epigenetic chromatin modification of cells with high precision.
A new Tool for Epigenetic State Control
The optical tool takes the customizable DNA binding domain as the core of genome engineering applications. The binding domain was combined with photosensitive cryptochrome 2 protein from Arabidopsis and its interacting partner CIB1.
Blue light irradiation triggers the conformational change of CRY2, and then the CIB1 effector is recruited to the target site to mediate transcriptional regulation. The light kit can be easily customized to target many endogenous genomic sites and can be activated in a few minutes with reversibility.
The optical tool can be used in mouse neurons to mediate the reversible regulation of mammalian endogenous gene expression and target epigenetic chromatin modification.
Achievable Missions at Creative BioMart
Creative BioMart has mastered and developed a variety of optical switching systems for life science applications.
Customized Epigenetic States Tool Design
There can be a variety of DNA binding domains anchored to CRY2, such as zinc finger protein or RNA guided DNA binding domains of cas9 based nucleic acid decomposition inactivation mutants.
AVV Vector Design and Delivery
In order to apply this tool to neurons, we applied an adeno-associated virus (AAV) - based vector for gene transfer and simplified AAV production process. The single stranded DNA based genome of AAV is less susceptible to recombination, which provides an advantage over lentivirus vectors.
AVV Transfection and Transfection Effect Determination
- We will carry out the following steps to determine the effect of vector construction and transfection
- Primary neuron transduction and light stimulation experiment
- Immunohistochemistry of primary neurons
- Western blot
- Immunohistochemistry of brain sections
- Light stimulation and mRNA level analysis
- Chromatin immunoprecipitation
Why Choose us
- Creative BioMart has been upgrading and innovating existing technologies to ensure satisfactory delivery time and results.
- We provide cost-effective one-stop service, and the whole experimental process is transparent and verifiable.
- We provide customized services for each customer and formulate experimental schemes according to customer requirements.
- We have an advanced technical team and experimental platform, and all technicians have been strictly trained and screened to ensure the most accurate experimental results.
- We strictly keep business secrets and never disclose customer information.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
