C. elegans for In Vivo Toxicity Testing
Why Use C. elegans?
Caenorhabditis elegans is a small nematode that can be maintained at low cost and treated using standard in vitro techniques.
Unlike toxicity testing using cell cultures, Caenorhabditis elegans toxicity assay provides data from an entire animal with complete and metabolically active digestive, reproductive, endocrine, sensory and neuromuscular systems.
The toxicity grading screening of Caenorhabditis elegans has repeatedly proved that the LD50 grading of rats is as predictive as that of mice. In addition, many examples of protective toxic effects have been noted between Caenorhabditis elegans and mammals.
Larval Growth Assay
| Content |
A highly sensitive assay that can detect small disturbances in the developmental pathway. We can ensure that the negative test compound or treatment will not adversely affect the development of downstream detection or other systems. |
| Objective / results |
Worms are very sensitive to developing toxins. Using high-resolution images, we can accurately measure the size of hundreds of worms during development. |
| Methods |
To determine whether conditions or mutations affect the development of Caenorhabditis elegans, about 50 age synchronized first stage larvae (L1) grew on a standard plate with a bacterial food source. Worm size and life stage were evaluated 72 hours after plating. |
| Data collection |
Data on worm size (measured in length and area) were collected at different life stages including larval stages (L2, L3, L4) and / or young adults. |
Egg Viability Assay
| Content |
A highly selective assay for identifying compounds leading to egg viability has a higher probability of true positive and may be toxic to mammals. We measured the percentage of surviving offspring produced by drug treated worms. |
| Objective / results |
Mutations and compounds can affect the viability of animals. By quantifying embryonic viability, this assay can reveal the developmental effects of mutation and combination therapy. |
| Methods |
Adult Caenorhabditis elegans can lay eggs on a plate for 2 hours. Transfer a certain number of embryos to a new plate. After incubation at 20 ℃ for 24 hours, the number of non hatched embryos was calculated. |
| Data collection |
Embryo lethality is calculated by dividing the number of non hatched embryos by the total number of inoculated embryos. Test three repetitions. |
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