It is a Cre/Tet dependent photogenetic line.
The channelrhodopsin-2 cDNA sequence of Chlamydomonas reinhardtii was modified to include codons optimized for mammalian expression and function acquisition sequence replacement in order to produce greater stationary photocurrents. The optimized channel rhodopsin-2 sequence was fused with the enhanced yellow fluorescent protein sequence and inserted downstream of the mouse tet promoter.
After the CRE mediated stop box is removed, they can be used to generate tet off/tet on mutant animals with conditionally (inducible/reversible) expression of the modified Channelrhodopsin 2 -EYFP fusion protein. Subsequent irradiation of cells with blue light leads to reversible stimulation of action potential discharge/neural activity in these cells.
The modified ChR2 EYFP fusion protein was expressed in cells/tissues when fed with other mice expressing Cre recombinase and tetracycline controlled trans activator protein (tTA) or reverse tetracycline controlled trans activator protein (rtTA). After the induction of fusion protein, irradiation of neurons with blue light (~ 450-490 nm) will lead to rapid and reversible stimulation of strong action potential discharge activity in these cells.
Opsin is a retinal binding protein that provides light dependent ion transport and sensory functions for halophilic bacteria. The optimized opsin acts as a blue light driven cation channel to depolarize cells and generate action potential. Therefore, irradiation of expressed neurons with blue light (450-490 nm) will lead to rapid and reversible stimulation of action potential discharge/neural activity in these cells.
At present, Creative BioMart has developed a variety of mouse strains expressing Channelrhodopsin, halorhodopsin and Archaerhodopsin respectively, which facilities the application of optogenetic. We have complete animal maintenance facilities to ensure animal health and animal welfare.
Expressed Gene: Yellow Fluorescent Protein (YFP) from jellyfish, Channelrhodopsin from Chlamydomonas.
Genotype: Inquiry
This mouse can be used to support research in many areas including
| Research Tools | Neurobiology Research |
|
|
Creative BioMart has strict gene quality control and mutation genotyping procedures. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheet. Therefore, we cannot guarantee that the phenotype of the strain will meet all expectations.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
Reference
