Drosophila Model for Optogenetic Research

Drosophila melanogaster is a useful model organism in a wide range of biological research from genetics to ecology. Drosophila is an extremely useful tool for studying various biological processes. It has low redundancy in Drosophila genome. Moreover, a large number of biological processes between Drosophila and mammals are highly conservative, and 75% of the known human genetic disease genes have homology in Drosophila. Creative BioMart has been working hard to develop Drosophila models that adaptive to optogenetics research. We provide customers with customized Drosophila model construction services.

Service and Core Facility

Drosophila is simple and cheap to raise in the laboratory. It can produce a large number of offspring, and the generation time is short (about 10 days). In addition, as an invertebrate, fruit fly is a morally ideal animal model. Our services include

Creating transgenic fruit flies

Creative BioMart provides plasmid generation guidance for the creation of transgenic fruit flies. We helped inject recombinant DNA into embryos and identify transgenic Drosophila lines. Thereafter, we provided guidance for mapping transgenes by cross protocol and / or reverse PCR.

Consultation and guidance on experimental setup

We provide guidance for the experimental setup of photogenetics, such as selecting appropriate fly lines for tissue-specific or ubiquitous target gene knockout / expression, hybridization scheme, fly phenotype, etc.

Maintenance of fly strains

We provide assistance in selecting and ordering Drosophila strains, including mutants, transgenic lines, RNAi, etc., and maintain them in the facility.

Fruit fly core facilities

The workstation is equipped with a stereomicroscope, an external light source and a humidified CO2 system for anesthetizing flies.
A flying food kitchen for preparing food and a refrigerator for storing food vials.
Isolation room for fly line and infection test from outside the facility. The quarantine room has two microscopes with carbon dioxide system.
Humidity and light controlled fly incubator at different temperatures (18 ° C, 25 ° C, 26 ° C, 29 ° C).

Experimental Equipment Setup

Stereo microscope for fly / larva imaging, including stereo microscope + imaging system, can be used for teaching and taking high-resolution color photos of flies and / or their anatomical tissues
Inverted microscope for imaging fly samples prepared on microscope slides
Stereo microscope for fluorescence sorting / imaging video imaging device for behavior analysis
Fruit fly activity monitor and incubator
Temperature adjustable incubator for hypoxia study, including incubator with adjustable O2 ratio

What is available at Creative BioMart?

Optogenetic requires cell specific gene expression to manipulate the neural activities of specific neuronal groups. In Drosophila, transgenic methods are widely used for this purpose.

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.