Cyanobacteria are considered to be ideal hosts for the production of biofuels or biomaterials, mainly because they can directly convert carbon dioxide into target substances, requiring only sunlight, water and some inorganic compounds. Because most Cyanobacteria are transformable, they can be genetically engineered to efficiently produce biofuels and biomaterials.

Synechocystis sp. PCC6803 is a kind of cyanobacteria. Creative BioMart optimizes a green light regulated gene expression system, which can be used for the engineering production of biomaterials and biofuels. The engineered green light gene expression system will be a useful genetic tool to control gene expression in the biological process of emerging cyanobacteria. The engineered green light gene expression system will be a useful genetic tool to control gene expression in the biological process of emerging Cyanobacteria.

How it Works?

The green light sensing system consists of green light sensing histidine kinase CcaS, homologous response regulator CcaR and cpcG2 (pcg2) promoter. CCAs and CCAR act as genetic controllers to activate the gene expression of PCG2 under green light irradiation.

Technical Advantages

Green light can effectively regulate gene expression in cyanobacteria and maintain sufficient growth conditions with red light.

We introduced a powerful SD like sequence to enhance gene expression. Under green light illumination, the constructed engineering green light sensing system has about 30 times higher protein expression than the wild-type promoter with high on / off ratio.

In the application of biofuel production, different RBS with different intensities will help to optimize the expression level of enzymes in the biosynthetic pathway to avoid unwanted by-products.

Our Service

• Codon optimization

It involves gene synthesis, vector construction, gene guaranteed transcription, mRNA translation, post-translational modification and so on.

• Vector construction and target gene subcloning.

A variety of prokaryotic expression vectors are available for selection.

• Screening of expressed strains and small amount induction detection of proteins.
• Optimize the expression scheme of the protein, and express and purify the protein in large quantities.
• Protein folding and modification.
• Protein identification.
• Proteins were identified by qualitative or quantitative analysis.

Service Advantages

• The success rate of the project is greater than 95%.
• One stop technical service
• Codon optimization: comprehensively consider more than 20 factors in the process of gene synthesis, vector construction, gene transcription, mRNA translation and modification.
• Large scale preparation and fermentation: we have 10L, 100L, 500L and 1000L fermentation tanks, which can deliver gram grade high-purity target protein to customers in a short time.

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

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