Application of Knock-out Model

• Use the customized knockout mouse model to gain insight into key genetic mechanisms and accelerate your research.
• Discover gene functions and explore cellular processes
• Deeply understand the pathogenesis and prevention of diseases
• Simplify pharmacodynamic evaluation in drug discovery

Gene Knock-out Models Include

• Conventional gene knockout
• Conditional gene knockout
• KO first

You can contact Creatice BioMart's technical consultant to design and customize your gene knock out mouse model for you.

Conventional Gene Knock-out

Establish a conventional gene knockout (KO) mouse model, so that the target gene function you are interested in is permanently lost in all cells of the mouse and in the whole life process.

Through CRISPR gene editing technology, it generally takes 4-6 months to construct a conventional gene knockout mouse model.

According to the gene sequence design, sgRNA and cas9 mRNA are synthesized and injected into mouse fertilized eggs together. Cas9 nuclease, sgRNA and genomic target sequence bind and cut double stranded DNA, resulting in frameshift mutation or fragment knockout of target gene through non homologous end junction (NHEJ) repair pathway, so as to realize gene knockout.

Conditional gene knockout

Conditional knockout (CKO) can be used to knock out the target genes you are interested in through time or tissue specificity, so as to achieve more accurate gene knockout and conduct more targeted research.

Conditional gene knockout is mainly realized by chromosomal site-specific recombinase systems CRE loxP, FLP FRT and dre Rox. The most commonly used system is CRE loxP system. A loxP sequence is placed at both ends of a target DNA sequence to be knocked out to obtain Flox (flagged by loxP) mice. By mating Flox mice with tool mice specifically expressing CRE, mice with tissue or cell specific knockout of target genes can be obtained.

Through CRISPR gene editing technology, it generally takes 6-9 months to construct a conditional gene knockout mouse model.

KO First

Ko first (conditional ready) is a multi-purpose model, which is similar to the conditional knockout strategy. LoxP sites with the same direction are placed on both sides of the target fragment, and a SA IRES reporter fragment with FRT sites on both sides is placed in the intron at the 5 'end of the target fragment.

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