Figure 1: Cholinergic neurons and networks in the rodent CNS. (Perez-Lloret, S., 2016) The channelrhodopsin-2 cDNA sequence from the Chlamydomonas reinhardtii was modified to contain codons optimized for mammalian expression and functional acquisition sequence substitution, aiming to cause greater fixed photocurrent. The optimized channelrhodopsin-2 sequence was fused with the enhanced yellow fluorescent protein sequence and inserted downstream of the mouse acetylcholine transferase (ChAT) promoter.
Bacterial opsin is a retinal binding protein that provides light dependent ion transport and sensory functions for halophilic bacteria. The optimized opsin acts as a blue light driven cation channel to depolarize cells and cause action potentials. Therefore, irradiation of expressed neurons with blue light (450-490 nm) will lead to rapid and reversible stimulation of action potential discharge/neural activity in these cells. Hemizygous mice are viable and fertile with normal life expectancy. Yellow fluorescence was observed under fluorescence microscope after expression activation.
By adding or removing light to quickly control motor behavior, the transgenic mice can be used to study the neural circuit/connectivity after illumination in vitro and in vivo, for fluorescent labeling of cholinergic neuron groups, or for studying the consequences of hyperactive cholinergic signals in information processing, memory, behavior and physical health.
At present, Creative BioMart has developed a variety of mouse strains expressing Channelrhodopsin, halorhodopsin and Archaerhodopsin respectively, which facilities the application of optogenetic. We have complete animal maintenance facilities to ensure animal health and animal welfare.
Expressed Gene: Yellow Fluorescent Protein (YFP) from jellyfish, Channelrhodopsin from Chlamydomonas.
Site of Expression:
Mice showed strong expression of EYFP Cholinergic neurons in striatum, trochlear nucleus, medial habenular nucleus, interphalangeal nucleus and various brainstem motor neuron nuclei, cortex, hippocampus and other brain regions.
Genotype: Inquiry
This mouse can be used to support research in many areas including
| Research Tools | Neurobiology Research |
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Creative BioMart has strict gene quality control and mutation genotyping procedures. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheet. Therefore, we cannot guarantee that the phenotype of the strain will meet all expectations.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
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