Figure 1: Phototagging neuronal populations based on their genetic identity and projection targets during in vivo electrophysiological recordings. (Jennings JH, 2013) The transgenic mouse strain can express channelrhodopsin protein, and the use of mice does not depend on Cre recombinase.
This transgenic hemizygous mouse is dynamic and fertile. Mammalian codon optimized Chlamydomonas reinhardtii composition derived channelrhodopsin 2 and yellow fluorescent protein (YFP) are expressed in the form of fusion protein. Compared with ChR2, the optimized opsin sequence aims to produce greater fixed photocurrent. Bacterial opsin is a retinal binding protein that provides light dependent ion transport and sensory functions for halophilic bacteria. Opsin acts as a cation channel driven by blue light, depolarizing cells and causing action potentials. Therefore, irradiation of opsin expressing neurons with blue light (450-490 nm) will lead to rapid and reversible stimulation of action potential discharge/neural activity in these cells.
After activation, yellow fluorescence is visible under fluorescence microscope. The transgenic mice of this strain can not only be used to quickly control motor behavior by adding or removing light, for in vitro and in vivo studies of neural circuit/connectivity after illumination, but also for fluorescent labeling of neuronal populations.
At present, Creative BioMart has developed a variety of mouse strains expressing Channelrhodopsin, halorhodopsin and Archaerhodopsin respectively, which facilities the application of optogenetic. We have complete animal maintenance facilities to ensure animal health and animal welfare.
Expressed Gene: Yellow Fluorescent Protein (YFP) from jellyfish, Channelrhodopsin from Chlamydomonas.
Site of Expression:
Mice showed strong expression of EYFP in olfactory bulb (glomerular and mitral valve cell layer), thalamic reticular nucleus, superior colliculus, inferior colliculus, brainstem and cerebellar molecular layer. Moderate EYFP expression was found in cortex, hippocampus, thalamus and olfactory bulb granular cell layer.
Genotype: Inquiry
This mouse can be used to support research in many areas including
| Research Tools | Neurobiology Research |
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Creative BioMart has strict gene quality control and mutation genotyping procedures. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheet. Therefore, we cannot guarantee that the phenotype of the strain will meet all expectations.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
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