Strain for Smooth Muscle Related Research
Strain Construction
Chlamydomonas reinhardtii derived channelrhodopsin-2 (ChR2 or COP4) is a blue light driven cation channel that depolarizes cells and generates action potential. The calcium-translocating channelrhodopsin (CatCh) is a ChR2 variant.
Transgenic mice of this strain expresses calcium-translocating channelrhodopsin CatCh in smooth muscle cells (such as blood vessels, lungs and airways). Bacterial opsin is a retinal binding protein that binds photosensitive domain and ion channel or pump, providing bacteria with light dependent ion transport, membrane potential change and sensory function. We optimized CatCh. The optimized CatCh shows stronger Ca2+ permeability, resulting in faster response time and voltage response to light than wild-type ChR2 Sensitivity is about 70 times.
Strain Description
Irradiation of CatCh molecules with blue to green light in smooth muscle resulted in reversible light stimulation of channel opening, calcium influx and muscle contraction.
CatCh has high sensitivity to blue to green light. Therefore, irradiation of CatCh expressing cells with blue to green light (450-530 nm, maximum 474 nm) will lead to rapid and reversible stimulation of channel opening (e.g., calcium inflow and contraction of muscle, or strong action potential discharge activity in neurons, etc.). Because CatCh activation is possible under naturally occurring light intensity while maintaining high time accuracy, it may also be applied to visual restoration in gene therapy.
At present, Creative BioMart has developed a variety of mouse strains expressing Channelrhodopsin, halorhodopsin and Archaerhodopsin respectively, which facilities the application of optogenetic. We have complete animal maintenance facilities to ensure animal health and animal welfare.
Strain Details
Expressed Gene: Channelrhodopsin from Chlamydomonas reinhardtii
Expressed Site: Smooth muscle cells in mice, such as blood vessels, lungs, airways, bladder and intestine
Genotype: Inquiry
Strain Application
This mouse can be used to support research in many areas including
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Research Tools |
Cell & Developmental Biology Research |
- issue/Cell Markers: transplantation marker for embryonic and adult tissue
- Tissue/Cell Markers
- Tissue/Cell Markers: multiple
- Neurobiology Research
- cell marker
- lacZ Expression
- Cardiovascular Research
- Developmental Biology Research
- transplantation marker for embryonic and adult tissue
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- Channel and Transporter Defects
- Internal/Organ Defects
- Brain
- Mesodermal Defects
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| Neurobiology Research |
Other |
- Channel and Transporter Defects
- Optogenetic and Chemogenetic tools
- Channelrhodopsin expressing strains
- LacZ expression in neural tissue
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- Cardiovascular Research
- Heart Abnormalities
- Internal/Organ Research
- Lung Defects
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Please note that
Creative BioMart has strict gene quality control and mutation genotyping procedures. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheet. Therefore, we cannot guarantee that the phenotype of the strain will meet all expectations.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
