Strain for Vascular Related Research
Strain Construction
The calcium-translocating channelrhodopsin (CatCh) is a ChR2 variant. The transgenic mice of this strain expressed CatCh in vascular endothelial cells (such as heart, lung and brain). Bacterial opsin is a retinal binding protein that binds photosensitive regions and ion channels or pumps to provide bacteria with light dependent ion transport, membrane potential changes and sensory functions. We optimized the CatCh. Optimized CatCh shows stronger Ca2+ permeability, resulting in faster response time and voltage response to light, which is about 70 times more sensitive than wild-type ChR2.
Strain Description
Irradiation of CatCh molecules with blue to green light in smooth muscle resulted in reversible light stimulation of channel opening, calcium influx and vasoconstriction.
CatCh is highly sensitive to blue to green light. Therefore, irradiation of CatCh expressing cells with blue to green light (450-530 nm, maximum 474 nm) will lead to reversible light stimulation leading to channel opening, calcium influx and muscle relaxation.
At present, Creative BioMart has developed a variety of mouse strains expressing Channelrhodopsin, halorhodopsin and Archaerhodopsin respectively, which facilities the application of optogenetic. We have complete animal maintenance facilities to ensure animal health and animal welfare.
Strain Details
Expressed Gene: Channelrhodopsin from Chlamydomonas reinhardtii
Expressed Site: Vascular endothelial cells such as heart, lung and brain
Genotype: Inquiry
Strain Application
This mouse can be used to support research in many areas including
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Research Tools |
Cell & Developmental Biology Research |
- Tissue/Cell Markers: endothelial cells
- Tissue/Cell Markers: multiple
- Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
- Tissue/Cell Markers
- Diabetes and Obesity Research
- Neurobiology Research
- lacZ Expression
- Cardiovascular Research
- Developmental Biology Research
- Transplantation marker for embryonic and adult tissue
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- Internal/Organ Defects
- Brain
- Lung
- Heart
- Heart: vasculature
- Internal/Organ Research
- Lung Defects
- Heart Abnormalities
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| Neurobiology Research |
Other |
- Optogenetic and Chemogenetic tools
- Channelrhodopsin expressing strains
- Channel and Transporter Defects
- lacZ expression in neural tissue
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- Cardiovascular Research
- Vascular Defects
- Heart Abnormalities
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Please note that
Creative BioMart has strict gene quality control and mutation genotyping procedures. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheet. Therefore, we cannot guarantee that the phenotype of the strain will meet all expectations.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
Reference
- YamadaMager T, Wood PG, Bamberg E. Optogenetic Control of Ca2+ and Voltage-Dependent Large Conductance (BK) Potassium Channels. J Mol Biol. 2017 Mar 24;429(6):
