Figure: Different modes of collective cell migration (Olson, H, 2018)

Zebrafish (Danio rerio) is a translucent fish with good genetic characteristics. It is a popular model organism in optogenetics. Its transparency in embryonic and larval stages can be used to study living organisms through subcellular resolution. Due to its beneficial optical properties of zebrafish, it not only allows passive observation, but also allows the use of optogenetic tools to actively manipulate proteins and cells through light for the study of molecular mechanisms driving development and disease.

Creative BioMart has been able to provide our customers with zebrafish models adapted to optogenetic tools to study cell migration. At present, there are two kinds of optical switches that can be used to regulate cell migration, photoactivated Rac and channelrhodopsin (ChR). Based on the above switching system, we can provide the following zebrafish strains.

Zebrafish for Cell Migration Control

Cytoskeleton is the main contributor to cell migration and morphological changes. We can establish several optogenetic tools for customers to control cytoskeleton dynamics in a precise way, which provides the possibility to study the mechanism of migration.

Photoactivatable Rac for Cell Directional Movement Research

Mechanism of action: light-induced Ras-GTPase activity.

Application in zebrafish: control over neutrophil movement.

Photoactivatable Rac is formed by fusing a photosensitin derived LOV domain with Rac1 to spatially inhibit RAC function. Blue light irradiation can restore Rac activity and induce cell directional movement in vitro. In zebrafish embryos expressing PA Rac in neutrophils, laser irradiation can accurately regulate the directional migration of neutrophils. In this way, neutrophils can even be transferred from the wound, opening up new possibilities for the study of cell function in inflammation.

Channelrhodopsin (ChR)

Mechanism of action: cation-channel, modulation of cellular ion concentrations.

Application in zebrafish: perturbation of pigment migration.

In addition to controlling neuronal activity, ChR is also used to regulate cell migration. Through the tissue-specific expression of ChR2 (a blue light reactive cation channel protein), the pigmentation pattern of zebrafish is temporarily disturbed by light, which is due to the induced migration of pigment melanin groups.

Why Select Us?

• We have advanced zebrafish experimental core equipment.
• We have professional technical and R & D personnel.
• We provide customized one-stop service, and the experimental process is transparent and verifiable.
• We have professional customer service personnel to ensure 7 / 24 online and solve problems for you at any time.

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

Reference

1. Olson, H. M., & Nechiporuk, A. V. (2018). Using Zebrafish to Study Collective Cell Migration in Development and Disease. Frontiers in cell and developmental biology, 6, 83.

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