The ability to precisely control the location, quantity and timing of gene expression will have a transformative impact on the cell and developmental biology community.
In zebrafish and other multicellular model organisms, the current inducible gene expression system is very limited in space and time control. Time control is achieved by administration of small molecular compounds, such as tetracycline induced expression system or activation of heat shock promoter. In the case of small molecule dependent activation, time and penetration may be variable and difficult to control.
Spatial control is mainly achieved by using tissue-specific promoters, but this method is limited by reliable tissue-specific promoters and can not control at the sub-tissue level.
A promising technology for finer spatio-temporal control of gene expression is light-based transcriptional control using photogenetic transcriptional activators. The ideal zebrafish optogenetic system is genetically encoded, does not need complex optical systems or exogenous small molecules, has a wide range of induction, has fairly fast kinetic reversibility, and, importantly, has little toxicity.
Creative BioMart provides a variety of zebrafish model construction services for optogenetic technology, including but not limited to
We designed a blue light activated Light-Oxygen-Voltage domain (LOV) switching system for zebrafish. LOV protein dimerization occurs when irradiated with blue light. It not only has low toxicity, but also shows a wide range of induction, fine spatial accuracy and rapid kinetics.
We directly introduced the channelrhodopsin-2 variant into the adult zebrafish brain by herpes simplex virus type I virus (HSV-1) or electroporation, and produced a strong long-term expressed promoter.
CRISPR activation and photogenetic system were combined to regulate the transcriptional activation of zebrafish genes. Spatiotemporal control provides simplicity, flexibility and effectiveness for gene expression research.
Additionally, we also provide zebrafish strains construction services for non-neuromodulatory research, including but not limited to
Do you need any help? Here is an overview of non-neural optogenetic tools used in zebrafish that gathers the information you may need.
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