Zebrafish for In Vivo Toxicity
Creative BioMart provides services and expertise throughout the zebrafish workflow, including genetic modification, molecular characterization and behavioral testing. Our toxicity tests use automated and customized software for data collection and analysis. Our main testing services include:
Acute Toxicity and Teratogenicity Screening
| Content |
The acute developmental toxicity study of zebrafish can predict the mammalian exposure level related to embryotoxicity and teratogenicity. |
| Objective / results |
By testing a series of concentrations, we defined the concentrations of LD50, Noel and mnld. |
| Methods |
Our team tested the teratogenicity of the compound on a series of morphological development endpoints and two behavioral results of zebrafish embryos between 24 and 120 hours after fertilization. |
| Data collection |
All toxicity screening data are collected in an automated high-throughput workflow, which combines the collected experimental endpoints into the development hazard profile report, while retaining individual animal level details and providing original data files for user analysis. |
Morphological Endpoints
Assessed at 24 hpf : mortality, developmental progression, spontaneous movement, and spinal cord development were assessed.
Evaluated at 120 HPF: total mortality, body axis (body axis nonlinearity, eye edema, yolk sac edema and pericardial edema and brain malformation), ear vesicle, pectoral fin, caudal fin, pigmentation, blood circulation, swim bladder formation, absence or non inflation.
Behavioral Response
Embryonic photokinetic response (EPR) behavior. EPR determination is used as an early sensor for 22-24 HPF chemical developmental hazards.
Larval photokinetic response (LPR) behavior. LPR can reveal the neuromuscular effects of compounds that may not be detected by morphological malformations.
Other Zebrafish Testing Services
| Embryo acute toxicity test |
Determine the acute or fatal toxicity of chemicals to fish embryos. |
| Behavioral phenotype of zebrafish |
Evaluate light motor response, measure anxiety behavior, or understand learning and memory mechanisms. |
| Early life stage toxicity test |
Determine the lethal and sublethal effects of chemicals on the early life stage of the tested fish. |
Custom test project. Still can't find what you're looking for? We can work with you to create customized in vivo test solutions to provide you with the answers you need.
Why use Zebrafish?
Zebrafish is increasingly regarded as an indispensable tool in toxicity testing. For companies seeking to test for acute toxicity and teratogenicity, the detection of zebrafish larvae can provide predictive answers in just a few weeks.
Studies have confirmed that the toxicity characteristics of mammals and zebrafish are surprisingly similar. In addition, zebrafish organs are similar to humans in cell composition, function, signal transduction, response to injury and cellular processes mediating organ specific diseases. Many genes are highly conserved between humans and zebrafish, making them useful systems for studying the basic mechanisms of specific organ diseases, such as heart, liver or nervous system diseases.
Zebrafish developed rapidly. All of their major organ systems are fully formed in the early larval stage, making it a recognized standard model for evaluating organ toxicity. Even in the early stage, zebrafish showed consistency at the level of metabolic pathway, confirming its practicability as a model for screening the potential toxicity of compounds to main organs.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
