As a pioneer in the biotechnology industry, Creative BioMart has the ability to provide a variety of gene editing services based on zebrafish in vivo, including but not limited to mutation, labeling and transgene using CRISPR and Tol2 genome editing tools. We can insert the optogenetic elements you are interested in into the genome of zebrafish through this gene editing service.
Our scientific team has many years of comprehensive zebrafish gene editing experience. Our service can significantly shorten your experimental design time, and can be customized according to different experimental requirements. Our goal is to help you advance the experiment, speed up the time from project creativity to project implementation, and choose the most suitable way for you.
We offer comprehensive service package deals with the whole process of design, validation, injection, feeding and strain screening to provide stable transgenic zebrafish lines.
| Items | Description |
| Project design | Select the best guiding RNA around the site to be edited. |
| sgRNA detection | In vivo cleavage analysis was performed by PCR / sequencing to determine the most effective sgRNA. |
| Donor homology design | The repair template is designed around high-quality sgRNAs. |
| Injection | Skilled syringe for editing reagents for rigorous in vivo testing. |
| Clutch verification | Injected embryos were sequenced and evaluated by PCR and / or editing. |
| Germline verification | Verification of germline editing. |
We offer a variety of service combinations to meet your laboratory needs and budget. Choices range from a quick, affordable custom injection mix to a comprehensive, complete build service package that allows you to customize the support you need to achieve your gene editing goals.
We use a collaborative approach to design the project with you to ensure that you get the zebrafish crossing that best suits your experiment.
Destroy or delete the complete domain in the gene coding sequence. It is often used to study gene function.
Precise nucleotide changes were introduced at the target site. It is usually used to understand gene function or more accurately simulate human diseases.
Mark your favorite gene at the local locus of the zebrafish line. Select from a large number of fluorescent proteins and epitope tags.
LoxP sites are inserted on both sides of the region to be deleted in zebrafish for tissue-specific or temporary deletion of genes of interest.
Large cargo or expression cassettes were inserted into zebrafish genome using Tol2 transposon based system for tissue-specific reporter gene expression or regulation of gene expression.
In addition to the gene editing services, we also offer
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
