Zebrafish Knock-in Tagging Services
Creative BioMart marks your favorite genes at natural sites of zebrafish lines through CRISPR / cas9 gene editing technology. Select from a large number of fluorescent proteins and epitope tags.
Fluorescent labeling is often used to help visualize the spatiotemporal dynamics of protein products of a given gene in living (sometimes fixed) animals.
Epitope markers are most commonly used to promote biochemical research, especially protein-protein interaction, which requires the existence of easily identifiable epitopes or sequence features for chemical modification or separation.
Available Tagging at Creative BioMart
- Knocking in a fluorescent tag at the native locus.
Use fluorescence to observe when and where genes of interest are expressed. Fluorescent markers encoded by genes (GFP, mCherry, etc.) can be inserted into the frame to label the N-terminal or C-terminal of the protein.
- Knocking in a protein tag.
Gene coding tags (FLAG tag, HA tag, poly (His) tag, etc.) can be inserted into the frame to mark the N-terminal or C-terminal of the protein. The proteins can then be observed by Western blotting, immunofluorescence and immunoprecipitation using commercially available antibodies.
Fluorescent Tagging or Epitope Tagging in Zebrafish
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Fluorescent Tagging |
Epitope Tagging |
| Tagging applications |
In vivo imaging studies, fate maps, single cell analysis (e.g., RNA SEQ) |
Biochemical studies (such as pull-down test) and immunohistochemistry in fixed tissue samples |
| Features |
It can help to visualize the spatiotemporal dynamics of protein products of a given gene in living (sometimes fixed) animals. |
- The size is relatively small, which can promote the visualization or separation of protein expression,
- Reduce opportunities to disrupt endogenous expression levels or protein transport dynamics.
- Relatively powerful and inexpensive commercially available antibodies can also be used to promote the visualization or biochemical modification of proteins in fixed tissues.
|
| Limitations |
Because the fluorophore used is relatively large, it may interfere with gene function. |
Insufficient verification. |
One-stop Tagging Service
Select this service if you want us to provide one-stop service that have been verified by stable sequences. You will get
- F0 injected embryo
- F0 injection embryo feeding service
- Strictly screen F0 injected adults for editing of germline transmission
- Sequence conformation and reproduction of F1 heterozygous animals
During the experiment, we will test whether your sgRNAs have the ability to guide cas9 cutting. We will also identify SNPs and polymorphisms in loci that may affect homologous directed repair. Embryos were injected with two different sgRNA / cas9 double stranded bodies, and we determined the cutting efficiency of each sgRNA. If neither sgRNA could guide successful cleavage, a third sgRNA was injected and tested.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
