The ability to control transgene expression in plant system is very important for the analysis of complex regulatory systems and metabolic pathways, especially the study of genes that have harmful effects on plant growth and development and therefore cannot be constitutively expressed. In addition, inducible transgene expression can be used to produce therapeutic proteins in plant cell culture to limit protein production to the growth period with optimal biosynthetic ability. Several transgenic expression systems that can be controlled by chemicals have been developed. However, these systems are controlled by small molecules and are inherently limited by these chemicals. Due to the diffusion of inducers, the temporal resolution of gene expression in chemical control system is poor. In addition, many inducers have pharmacological activities. Therefore, it is not desirable to add them to plant cell cultures for the production of biological drugs.
Compared with small chemical inducers, light with cell compatible wavelength is not limited by regulation in protein biological production, and can be transmitted with high time resolution. Therefore, light is a very potential inducer in plant system.
Creative BioMart has developed a powerful plant transformation platform. Based on this platform, we can construct light induction systems on Arabidopsis, tobacco and Sinorhizobium meliloti respectively. The platform is equipped with professional molecular biology laboratories, cutting-edge equipment and excellent experts. We are committed to becoming a leader in plant engineering and crop improvement, and contribute to the development of plant related research.
We offer Arabidopsis thaliana system that respond to the stimulation of red / green light as well as a Arabidopsis thaliana system under white light.
The implementation of the system in the instantaneous determination of tobacco protoplasts leads to the strong induction of red light (660 nm) and can immediately return to the off state by subsequent far red light (740 nm).
Sinorhizobium meliloti is a gram-negative α-Proteus, which can fix atmospheric nitrogen during symbiosis with some host plants (such as alfalfa). We constructed a blue light induced gene expression system in S. meliloti strain Rm8530.
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