Plant transgenic operation refers to the transfer and integration of DNA sequences or plasmids into plant genome through genetic engineering to obtain new plants. Plant transgenic operation is a tool for character introduction and selection in the field of modern agricultural breeding. It is also an indispensable method for further study of plant growth and development.

Creative BioMart has developed a powerful plant transformation platform for gene manipulation of a variety of food crops, cash plants and model crops. The platform is equipped with professional molecular biology laboratories, cutting-edge equipment and excellent experts. We are committed to becoming a leader in plant engineering and crop improvement, and contribute to the development of plant related research.

Plant Genetic Transformation

• Transformation systems of common plants such as cucumber, melon, soybean, tomato, tobacco, Arabidopsis, cotton, rape, rice, potato and corn.
• CRISPR / cas9 gene editing, Arabidopsis, tomato, rice, tobacco and so on can achieve nearly 70% mutation rate.

Vector Construction

• Microbial expression / knockout vector.
• Plant expression / silencing vector.
• Plant VIGS vector.
• Plant CRISPR / cas9 gene editing vector.
• Subclonal vector.

Figure: Editing plant genomes with CRISPR/Cas9 (Belhaj K, 2015)

Molecular Cloning

• Gene RACB.
• Gene cloning / reverse transcription PCR.
• Gene directed mutation.
• Transformed Escherichia coli / Agrobacterium.

Biological Detection

• Fluorescence quantitative PCR.
• Gene marker detection.
• Identification of secondary metabolites.

Basic Service Process

• Communicate with customers.
• Design experiment.
• Sign contracts and confidentiality agreements with customers.
• Update project status.
• Final report containing organized data and analysis.
• Basic service process.

Advantages of Creative BioMart Transgenic Operation Service

• Creative BioMart Efficient and stable conversion system.
• Multiple delivery strategies for multiple species and genotypes.
• The experimental cycle is short, and conventional plants only take 3-9 months.
• We have many years of experience in plant genetic transformation systems.

Related Services

Did not find your intended services? In addition to the Plant Genetic Transformation Services, we also offer

• Arabidopsis thaliana Optogenetics
• Nicotiana tabacum Optogenetics
• Sinorhizobium meliloti Optogenetics

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

Reference

1. Belhaj K, Chaparro-Garcia A, Kamoun S, Patron NJ, Nekrasov V. Editing plant genomes with CRISPR/Cas9. Curr Opin Biotechnol. 2015 Apr;32:76-84.

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