Green Light Control Arabidopsis thaliana System

Arabidopsis thaliana is a popular model organism in plant biology and genetics. It is the first plant to sequence its genome. It is also a popular tool for molecular biology to understand many plant traits, including flower development and light sensing.

One opportunity to cultivate the potential of photogenetics in plants is to design green light response gene switches, because plant photoreceptors show reduced activity in this part of the spectrum. Creative BioMart provides a protocol of the first synthetic optical response system and provide construction services. The system is used to target and control gene expression in Arabidopsis cells. The system responds to the green spectrum with minimum activity of plant photoreceptors.

How does it Works?

Green Light Control Arabidopsis thaliana System

The Overview of the Service Process

Sign service contract
The client transfers the target gene and makes payment
We carried out transgenic experiments
Deliver the experimental results to the customer, and the customer checks and accepts them

Arabidopsis thaliana Transgenic Service

Service Content

Vector construction
Plant culture of Arabidopsis thaliana
Protoplast isolation and polyethylene glycol-mediated transformation
Selection of t0 generation seeds
Pure line screening and PCR detection of foreign gene
Illumination treatment and reporter assay

Service Description

Receptor materials: Columbia and other varieties are receptor materials.
Number of experiments: each transgenic vector was transformed into one receptor material once.

Provide Results

Some pictures of genetic transformation and screening.
Agarose gel electrophoresis of PCR gene in T0 transgenic seedlings.
T1 generation transgenic seeds: T1 generation seeds of 5 or more lines were obtained in each transformation experiment

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