Strains Expressing Archaerhodopsin
Strain Construction
The modified Archaerhodopsin-3 (Arch) from halorubrum sodomense was fused with enhanced green fluorescent protein (EGFP) and inserted into the endogenous locus of transgenic mice. The strain is Cre recombinase dependent. When mating with mice expressing Cre recombinase, the resulting offspring will delete the stop box in CRE expressing tissues; Results in the expression of Arch EGFP fusion protein. The strains of hemizygous mice were dynamic, fertile, normal in size, and did not show any obvious physical or behavioral abnormalities.
Irradiation of these cells with yellow green light (~ 575 nm) resulted in reversible inhibition of action potential discharge/neural activity in these cells. These transgenic mice can be used for optogenetic reseArch to express an inhibitory opsin, which can effectively inhibit the activity of cortical neurons (and other excitable cell types, such as muscle cells and immune cells). Compared with the improved Arch, the optical sensitivity is increased by more than 3 times, which is equivalent to an increase of more than 200% in the volume of brain tissue treated by a typical single optical fiber.
Strain Description
After exposure to Cre recombinase, irradiation of Arch expressing neurons with yellow green light (~ 575 nm) resulted in reversible inhibition of action potential discharge/neural activity in these cells.
Compared with wild-type Arch, the improved Arch can mediate photocurrent with similar maximum amplitude, but compared with Arch, the photosensitivity is more than three times higher, which is equivalent to more than twice the volume of brain tissue treated by a typical single optical fiber. The expression of modified Arch on neuronal membrane and axon is good, which may support improved neural pathway silencing.
At present, Creative BioMart has developed a variety of mouse strains expressing Channelrhodopsin, halorhodopsin and Archaerhodopsin respectively, which facilities the application of optogenetic. We have complete animal maintenance facilities to ensure animal health and animal welfare.
Strain Details
Expressed Gene: GFP, Green Fluorescent Protein, Archaerhodopsin-3 (Arch) from halorubrum sodomense
Expressed Site: Expresses extremely well on the membranes of neurons, including good expression on axons.
Genotype: Inquiry
Strain Application
This mouse can be used to support research in many areas including
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Research Tools |
Neurobiology Research |
- Cre-lox System
- Fluorescent Proteins
- Diabetes and Obesity Research
- Reproductive Biology Research
- Transplantation marker for embryonic and adult tissue
- Genetics Research
- Tissue/Cell Markers
- Mutagenesis and Transgenesis
- Tissue/Cell Markers: Cre-lox System
- Mutagenesis and Transgenesis: Cre-lox System
- Tissue/Cell Markers: cell marker for bone marrow transplantation
- Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
- Developmental Biology Research
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- Optogenetic and Chemogenetic tools
- Archaerhodopsin expressing strains
- Cre-dependent optogenetic tools
- Fluorescent protein expression in neural tissue
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Please note that
Creative BioMart has strict gene quality control and mutation genotyping procedures. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheet. Therefore, we cannot guarantee that the phenotype of the strain will meet all expectations.
Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.
