Gene knockout is a common tool for biologists to understand gene function. When genes are deleted, examining phenotypes can reveal the role of genes in organisms.

Using CRISPR / cas9 in zebrafish, we can destroy the complete regulatory domain or coding sequence of the gene (KO is invalid), and even remove the entire functional domain or coding sequence according to the gene size (KO deletion).

Creative BioMart has fully understand the different needs of customers in different experimental stages, so we have designed different service packages according to different situations. You can choose according to your needs, or you can choose with our help after consulting us

Service Package 1

If you want us to select your sgRNA site, have tested your sgRNA in vivo, or want CRISPR knockout, please select this service. We will design customized gene editing injections for you, and you can develop your favorite detection / screening scheme. You will get

• Develop genome editing strategy in consultation with genetic engineers
• Computer design of all reagents, including sgRNA sites and donor homologous templates
• Sequence files of donor homologous constructs and edited loci
• Mixture for injection
• Injection dyes are used for early visualization of injection success
• Detailed description of the hybrid reconstruction and injection scheme

Service Package 2

Select this service if you want to inject and filter your edits through PCR without having to worry about designing your own screening primers. This option includes our standard mixture and ready to use screening primers.

Service Package 3

Choose this service if you want us to use our rigorous internal testing process to ensure that your injection mixture is designed around efficient sgRNA sites. You will get

• Upgraded injection mixture
• Selection and testing of two sgRNAs for in vivo cleavage efficiency evaluation
• Donor homology designed around proven efficient sgRNA sites
• In vivo screening for potential polymorphisms in your target locus

Service Package 4

Select this service if you want to use a fully validated reagent for CRISPR typing in your laboratory and find your gene editing strain. This is the best custom injection mixing option for novice zebrafish crossing builders. You will get

• We validated the sgRNA injection mixture
• Complete in vivo evaluation of somatic editing efficiency
• Confirm your ability to integrate your edits into the zebrafish genome

Service Package 5

Select this service if you want to use a fully validated reagent for CRISPR typing in your laboratory and find your gene editing strain. This is a good choice for researchers who want to outsource part of their workload to the development of new production lines. You will get

• Complete and validated injection mixture
• Professional injection embryo
• Fully validated PCR primers and protocols for editing assays in your own laboratory

Service Package 6

Select this service if you want us to provide CRISPR knock in lines that have been verified by stable sequences. You will get

• F0 injected embryo
• F0 injection embryo feeding service
• Strictly screen F0 injected adults for editing of germline transmission
• Sequence conformation and reproduction of F1 heterozygous animals

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

Online inquiry