Strain Construction

The transgenic mice of this strain can be used for optogenetic research to express inhibitory opsin that effectively silences neuronal activity. The fragment formed by the fusion of halorhodopsin from natronomonas and enhanced yellow fluorescent protein (EYFP) was inserted downstream of the mouse thymocyte antigen 1 (Thy1) promoter of transgenic mice. The strains of hemizygous mice were dynamic, fertile, normal in size, and did not show any obvious physical or behavioral abnormalities.

Irradiation of halorhodopsin expressing cells with yellow to red light (~ 589-680 nm) resulted in reversible inhibition of action potential discharge/neural activity in these cells. These transgenic mice can be used for optogenetic research to express inhibitory opsin that effectively silences neuronal activity.

Strain Description

Bacterial opsin is a retinal binding protein that binds photosensitive domains and ion channels or pumps. It provides light dependent ion transport, membrane potential change and sensory function for bacteria. The mice of this strain used halorhodopsin as a yellow light driven chloride ion pump, which can lead to hyperpolarization and prevent action potential. Therefore, irradiation of halorhodopsin expressing neurons leads to rapid and reversible light inhibition of action potential discharge/neural activity in these cells.

These mice allowed in vivo light inhibition of cortical activity and limb movement. Halorhodopsin EYFP positive cells include neurons in various regions of amygdala, midbrain and lower brain stem, pyramidal cells in layer 5 of cortex, cells in anterior ventral nucleus of thalamus, granular cells in dentate gyrus and pyramidal cells in CA1 area of hippocampus, and mossy fibers in granular cell layer of cerebellum.

Irradiation of CatCh molecules with blue to green light in smooth muscle resulted in reversible light stimulation of channel opening, calcium influx and vasoconstriction.

At present, Creative BioMart has developed a variety of mouse strains expressing Channelrhodopsin, halorhodopsin and Archaerhodopsin respectively, which facilities the application of optogenetic. We have complete animal maintenance facilities to ensure animal health and animal welfare.

Strain Details

Expressed Gene: Halorhodopsin from natronomonas

Expressed Site: The pyramidal cells in the 5th layer of cortex, the cells in the anterior ventral nucleus of thalamus, the granular cells in the dentate gyrus, the pyramidal cells in the CA1 area of hippocampus, and the mossy fibers in the granular cell layer of cerebellum.

Genotype: Inquiry

Strain Application

This mouse can be used to support research in many areas including

Please note that

Creative BioMart has strict gene quality control and mutation genotyping procedures. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheet. Therefore, we cannot guarantee that the phenotype of the strain will meet all expectations.

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

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