The induced gene expression system in plants is essential for the study of cellular processes, the control of target gene expression with minimal or no interference to the development or growth process, and effective large-scale biopharmaceutical production. Spatial control of gene expression in plants has traditionally been achieved through the use of tissue-specific promoters. This leads to highly specific spatial gene expression. However, once such an expression box is implemented, the promoter can no longer be exogenous controlled.
Creative BioMart has developed a red / far red light induced synthesis system for Arabidopsis and provided construction services. This is a protocol of synthetic light induction system for targeted control of gene expression in plants based on plant photoreceptor phytochrome B and its interaction factor (PIF6). When the plant protoplast is irradiated by red light (660 nm), the synthetic toggle switch system is in the ON state and can return to the OFF state by subsequent far red light (760 nm).
After exposure to red light (660 nm), phyB changed its conformation to its active form (PFR), allowing binding to PIF6 and therefore recruiting trans activators to the smallest promoter. As a result, firefly luciferase was expressed. Under far red (760 nm) light irradiation, phyB was transformed into its inactive form (PR), and PhyB-PIF6 was dissociated, thus stopping the transcription of the reporter gene.
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