Creative BioMart can create a floxed allele for tissue-specific deletion or time control of the gene you are interested in. This is very useful in studying embryonic lethal genes or understanding the function of genes of interest in different tissues.

Technology Principle

CRISPR / cas9 is used to insert loxP site, which is located on both sides of the region to be deleted in zebrafish. Inserting two loxP sites can permanently remove the sequence of interest through the expression of Cre recombinase, and tissue or stage specific promoter expression can be used to accurately control gene function.

The line can then be hybridized with a cre line expressing Cre recombinase under a specific promoter, or injected with a plasmid containing Cre recombinase. Cre recombinase promotes the recombination of two loxP sites, and the region between sites is removed from the genome.

Conditional Mutagenesis by loxP Insertion

One of the more difficult aspects of biology is how to study the loss of function of basic genes. Now, conditional mutagenesis by inserting loxP into zebrafish is possible. We used sequential integration of loxP sequences to create a "floxed" gene for a large number of sequence deletions in the target locus.

Floxed Gene Service Options

Creative BioMart has fully understand the different needs of customers in different experimental stages, so we have designed different service packages according to different situations. You can choose according to your needs, or you can choose with our help after consulting us.

• Require for sgRNA site selection

Make sure that you have tested your sgRNA in vivo, or want CRISPR knockout and select this service. We will design customized gene editing injections for you, and you can develop your favorite detection / screening scheme. You will get

• Develop genome editing strategy in consultation with genetic engineers
• Computer design of all reagents, including sgRNA sites and donor homologous templates
• Sequence files of donor homologous constructs and edited loci
• Mixture for injection
• Injection dyes are used for early visualization of injection success
• Detailed description of the hybrid reconstruction and injection scheme

• Require for one-stop service

Select this service if you want us to provide one-stop service that have been verified by stable sequences. You will get

• F0 injected embryo
• F0 injection embryo feeding service
• Strictly screen F0 injected adults for editing of germline transmission
• Sequence conformation and reproduction of F1 heterozygous animals

Creative BioMart is always devote us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

Online inquiry