Strain Description

Bacterial opsin is a retinal binding protein that binds photosensitive domains and ion channels or pumps. It provides light dependent ion transport, membrane potential change and sensory function for bacteria.

The strain of hemizygous mice is dynamic, fertile, normal in size, and does not show any obvious physical or behavioral abnormalities. The fragment formed by the fusion of channelrhodopsin-2 (ChR2) derived from Chlamydomonas reinhardtii and yellow fluorescent protein (YFP) was inserted downstream of the mouse thymus cell antigen 1 (Thy1) promoter of transgenic mice.

Figure 1: Application of optogenetics in other neurological disorders (Ordaz, J. D., 2017)

These mice can be used for optogenetic studies, rapid control of motor behavior by increasing or eliminating light, in vitro and in vivo studies of neural circuits/connections after illumination, and fluorescent labeling of Thy1 expressing cells. ChR2 acts as a blue light driven cation channel, depolarizing cells and generating action potentials. Irradiation of ChR2 expressing neurons with blue light (~ 470 nm) resulted in rapid and reversible stimulation of action potential discharge/neural activity in these cells.

At present, Creative BioMart has developed a variety of mouse strains expressing Channelrhodopsin, halorhodopsin and Archaerhodopsin respectively, which facilities the application of optogenetic. We have complete animal maintenance facilities to ensure animal health and animal welfare.

Strain Details

Expressed Gene: Yellow Fluorescent Protein (YFP) from jellyfish, Channelrhodopsin from Chlamydomonas.

Site of Expression:

• Layer 5 cortical neurons.
• CA1 and CA3 pyramidal neurons of the hippocampus.
• Cerebellar mossy fibers, neurons in the thalamus, midbrain and brainstem, and mitral cells of the olfactory bulb.

Genotype: Inquiry

Strain Application

This mouse can be used to support research in many areas including

Please note that

Creative BioMart has strict gene quality control and mutation genotyping procedures. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheet. Therefore, we cannot guarantee that the phenotype of the strain will meet all expectations.

Why Choose Us

• Creative BioMart has been upgrading and innovating existing technologies to ensure satisfactory delivery time and results.
• We provide cost-effective one-stop service, and the overall experimental process is transparent and verifiable.
• We provide customized services for each client and formulate experimental schemes based on customer requirements.
• We have an advanced technical team and experimental platform, and all technicians have been strictly trained and screened to ensure the most accurate experimental results.
• We strictly keep business secrets and never disclose customer information.

Creative BioMart is always devoted us to provide high-quality and satisfactory service to our customers, if you are interested in our services or have some question, please feel free to contact us or make online inquiry.

Reference

1. Ordaz, J. D., Wu, W., & Xu, X. M. (2017). Optogenetics and its application in neural degeneration and regeneration. Neural regeneration research, 12(8), 1197–1209.

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